Journal:

Article Title: Secretion of Tumor Necrosis Factor-? from Human Placental Tissues Induced by Hypoxia-Reoxygenation Causes Endothelial Cell Activation in Vitro

doi:

Figure Lengend Snippet: a: Effects of increasing concentrations of placental-unconditioned or -conditioned medium (black bars, 1%; gray bars, 10%; open bars, 50%) on the viability of HUVECs after 24 hours of incubation. *, P < 0.05; compared to 1% placental-unconditioned or -conditioned medium. Hatched bar, cells grown in endothelial cell growth medium (EGM) alone. b: Effects of incubation period on the viability of HUVECs grown in a final concentration of 50% placental-conditioned medium. Gray bars, 24 hours of incubation; black bars, 48 hours of incubation. *, P < 0.05; **, P < 0.01, compared to cells grown in EGM alone. †, P < 0.05, compared to 24 hours of incubation. PCM, cells grown in placental-unconditioned medium; Normoxia, cells grown in conditioned medium from villous tissues kept at normoxia throughout; H/R, cells grown in conditioned medium from tissues subjected to hypoxia-reoxygenation. Data presented as mean ± SEM from four and three different experiments in (a) and (b), respectively.

Article Snippet: Culture of HUVECs HUVECs were obtained from BioWhittaker UK Ltd. (Wokingham, UK), and grown in complete endothelial cell growth medium (EGM; BioWhittaker) at 37°C with air/5% CO 2 .

Techniques: Incubation, Concentration Assay